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内容简介

While many excellent texts and monographs exist which cover various aspects of immunohistochemistry, the lack of a concise comprehensive guide to using the fielda (TM)s methods was a major motivation for the authors to write this book. The authors carefully selected the methods and easy-to-adopt protocols discussed. In addition to estabished techniques, special attention was p ai d to current developments in immunohistochemistry such as antigen retrieval, signal amplification, the use of epitope tags, multiple immunolabeling, and diagnostic immunohistochemistry. All procedures examined have been tested by the authors; many are routinely used in daily practice in their institute. Each chapter starts with a short introduction to the respective methoda (TM)s underlying principles before presenting step-by-step protocols and instructions, including precise recipes for all materials needed. Thus, the book is suitable for a broad audience, ranging from students and technical assistants to experienced researchers.

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